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Acta Academiae Medicinae Sinicae ; (6): 307-314, 2019.
Article in Chinese | WPRIM | ID: wpr-776033

ABSTRACT

Objective To describe the microbiological characteristics of ()CGMCC 12426 and determine and analyze its complete genome sequences.Methods strain CGMCC 12426 genomic DNA sequencing was performed on a single molecule real-time sequencing(SMRT)platform and the annotation was completed in the NCBI Prokaryotic Genomic Annotation Pipeline(pGAP).Results The complete genomic sequences of the released CGMCC 12426 consisted of a 4 138 265-bp circular chromosome and a 74 165-bp plasmid,which resulted in the prediction of 4581 genes including 4222 coding sequences,87 tRNAs,and 30 rRNAs(which included 5S rRNA,16S rRNA,and 23S rRNA).Conclusion The genome sequencing provided a basis for further investigations on the genetic background of and on the metabolic and regulatory mechanisms.


Subject(s)
Bacillus subtilis , Genetics , Genome, Bacterial , Plasmids , RNA, Ribosomal, 16S , Genetics , RNA, Ribosomal, 23S , Genetics , RNA, Ribosomal, 5S , Genetics , Sequence Analysis, DNA
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